Yes. The Desktop software has among other things the feature to upload/backup any open, locked or in-use file, ensuring that your business will run without interruption twenty four hours day, seven days a week.
To use this feature on Windows Vista, you'll have to run the Desktop Application as Administrator (by right clicking on the CompliantData icon and choose "Run as Administrator").
To use this feature on Windows XP, you have to make sure that the "Volume Shadow Copy" service is enabled.